IBlot Gel Transfer System (Invitrogen). Blots have been probed with anti-Atg5 (1:2000), anti-MREG (1:five hundred),
IBlot Gel Transfer System (Invitrogen). Blots have been probed with anti-Atg5 (1:2000), anti-MREG (1:five hundred), or anti-LC3 (one:a thousand) antibody and anti-actin (one:5000) to be a loading regulate. Membranes have…