AI site of pOK1/2 B [22] giving pOK1/2 B (ChlorR). Next, the attR1 site from
AI site of pOK1/2 B giving pOK1/2 B (ChlorR). Next, the attR1 site from pUC57 fragment A was cloned into this vector using BglII/NotI giving pBEG R1-ChlorR-R4. To make the…