By the Russian Foundation of Science (# 19-14-00110). Institutional Review Board Statement: Not applicable. Informed Consent Statement: Not applicable. Data Availability Statement: Not applicable. Conflicts of Interest: The authors declare that the analysis was conducted within the absence of any commercial or monetary relationships that may very well be Ziritaxestat custom synthesis construed as a prospective conflict of interest.
H OH OHmetabolitesArticleDynamics from the Metabolome of Aliinostoc sp. PMC 882.14 in Response to Light and Temperature VariationsDamien Le Moigne 1 , Justine Demay 1,2 , Anita Reinhardt two , C ile Bernard 1 , Sandra Kim Tiam 1,3 and Benjamin Marie 1, UMR7245 Mol ules de Communication et Adaptation des Micro-Organismes (MCAM) MNHN-CNRS, Mus m National d’Histoire Naturelle, CP 39, 12 Rue Buffon, CEDEX 05, F-75231 Paris, France; [email protected] (D.L.M.); [email protected] (J.D.); [email protected] (C.B.); [email protected] (S.K.T.) Thermes de Balaruc-Les-Bains, 1 Rue du Mont Saint-Clair BP 45, 34540 Balaruc-Les-Bains, France; [email protected] UMR5557 Laboratoire d’Ecologie Microbienne, Universitde Lyon, 43 bd du 11 novembre 1918, Villeurbanne, F-69622 Lyon, France Correspondence: [email protected]; Tel.: 33-1-4079-Citation: Le Moigne, D.; Demay, J.; Reinhardt, A.; Bernard, C.; Kim Tiam, S.; Marie, B. Dynamics in the Metabolome of Aliinostoc sp. PMC 882.14 in Response to Light and Temperature Variations. Metabolites 2021, 11, 745. https://doi.org/ ten.3390/metabo11110745 Academic Editor: Daniel Globisch Received: 23 August 2021 Accepted: 26 October 2021 Published: 29 OctoberAbstract: Cyanobacteria are microorganisms in a position to adapt to a wide selection of environmental conditions and abiotic stresses. They produce a large number of metabolites which will take part in the dynamic adaptation of cyanobacteria to a array of diverse light, temperature, and nutrient situations. Studying the metabolite profile is one particular way to understand how the physiological status of cells is related to their adaptive response. In this study, we sought to know how the diversity and dynamics with the complete metabolome depended on the growth phase and various abiotic aspects such as light intensity and temperature. The cyanobacterium, Aliinostoc sp. PMC 882.14, was selected for its huge number of biosynthetic gene clusters. One particular group of cells was grown under typical situations as a control, -Irofulven Description although other groups had been grown under greater light or temperature. Metabolomes were analyzed by mass spectrometry (qTOF-MS/MS) combined with untargeted evaluation to investigate metabolite dynamics, and important variation was discovered involving exponential and stationary phases, regardless of culture conditions. Within the higher light group, the synthesis of many metabolites, including shinorine, was induced although other metabolites, for example microviridins, had been synthesized beneath higher temperature conditions. Among extremely regulated metabolites, we observed the presence of mycosporine-like amino acids (MAAs) and variants of somamides, microginins, and microviridins. This study demonstrated the value of considering the physiological state of cyanobacteria for comparative global metabolomics and research on the regulatory processes involved in production of particular metabolites. Our outcomes also open up new perspectives around the use of organisms which include cyanobacteria for the targeted production of bioactive metabolites. Search phrases: cyanobacteria;.