Product Name :
Mouse anti Human CD45
Description :
| Clone BHPT-1 | Isotype IgG1 | Product Type Single-Color Reagent | Units 100 µg | Host Mouse | Species Reactivity Human | Application Flow Cytometry
Background :
Immunogen: Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient
Source :
Identification of all human leukocytes, lymphocytes, monocytes, polymorphonuclear cells, eosinophils and basophils in peripheral blood expressing the 180-220 kD M. W. T200 family surface antigen. CD45 is also present on thymus, spleen, tonsil and progenitor cells in bone marrow. Synonyms: CD45 <
Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Protein A/G Chromatography Concentration: See vial for concentration
Specificity :
Applications :
PBMC: Add 10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2° to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5+: As above, however use only 5 µl per test rather than 10 µl. Excites at 488nm and emits at 670nm. Store protected from light. Functional Analysis: Flow Cytometry Staining
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Room Temperature
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1 .Leukocyte Typing IV: White Cell Differentiation Antigens, Schwinzer R. Cluster report: CD45/CD45R, Knapp W., Dorken B., Gilks W.R., et al, eds. Oxford University Press; 1989:628-634 2. Association of CD2 and CD45 on human T lymphocytes. Schraven B., Samstag Y., Altevogt P., Meuer S.C., Nature 1990 May; 345(6270):71-4 Cy-5 + Portions of this product are manufactured under license from Carnegie Mellon University, U.S. Patent Number 5,268,486.
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