Product Name :
Mouse anti Human Cytochrome P450 3A5
Description :
| Clone F18 P3 B6 | Isotype IgG1 | Product Type Monoclonal Antibody | Units 200 µg | Host Mouse | Species Reactivity Human | Application ELISA Immunohistochemistry Western Blotting
Background :
Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminus of the human CYP3A5 protein and mouse myeloma Ag8563 cells.
Source :
CYP3A genes encode monooxygenases, enzymes which catalyze drug metabolism and the synthesis of cholesterol, steroids and other lipids. CYP3A (cytochrome P450, family 3, subfamily A), the most abundant p450 enzyme in human liver, is responsible for the metabolism of more than 50% of all clinical drugs. CYP3A members localize in organs that associate with drug disposition, including the liver, gastrointestinal tract and kidney. The CYP3A cluster consists of four genes: CYP3A43, CYP3A4, CYP3A7 and CYP3A5, and two pseudogenes: CYP3A5P1 and CYP3A5P2. The CYP3A cluster maps to gene locus 7q21.3-q22.1. Synonyms: CYP3A5; CYP450 3A5 (EC 1.14.14.1), Niphedipine oxidase, CYPIIIA5, P450-PCN3, HLp2 <
Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Protein A/G Chromatography Concentration: See vial for concentration
Specificity :
Applications :
Antibody can be used for Western blotting (1-2 µg/ml) and immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-5 µg/ml). When used in conjunction with CYP3A7 (Cat. No. X2048M), can differentiate between the CYP3A family members, 3A5 and 3A7. Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting Positive Control: Squamous oesophagus cancer
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Kumarakasingham, M., et al. (2005). Cytochrome p450 profile of colorectal cancer: Identification of markers of prognosis. Clin. Cancer Res. 11(10);3758-3765. 2. McCune, J.S., et al (2005). Contribution of CYP3A5 to hepatic and renal ifosfamide N-dechloroethlation. Drug Metab. Dispos. 33(7);1074-1081. 3. Kim, K.A., et al. (2007). Effect of polymorphic CYP3A5 genotype on the single-dose simvastatin pharmacokinetics in healthy subjects. J. Cin. Pharmacol. 47(1);87-93
Related websites: https://www.medchemexpress.com/antibodies.html
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