Product Name :
Mouse anti Human Estrogen Receptor
Description :
| Clone TE111.5D11 | Isotype IgG1 | Product Type Monoclonal Antibody | Units 100 µg | Host Mouse | Species Reactivity Chicken Cow Dog Hamster Human Mouse Pig Rabbit Rat Sheep | Application Chromatin Precipitation Functional Inhibition Gel Supershift Immunoprecipitation Western Blotting
Background :
Hybridoma produced by the fusion of splenocytes from mice immunized with the 35 kDa C-terminal fragment (aa 302-595) of human ER expressed in E. coli and mouse myeloma cells.
Source :
The estrogen receptor (ER) gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. <
Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Protein A/G Chromatography Concentration: See vial for concentration
Specificity :
Applications :
This antibody can be used gel supershift assays (neat concentration), immunoprecipitation on native and denatured lysate (2 µg/mg of protein lysate), Western blotting (1-2 µg/ml) and chromatin precipitation. Can also be used to block binding of estrogen to the estrogen receptor. Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting Positive Control: T47D Cells
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Abbondanza, C. et. al. ‘Characterization and epitope mapping of a new panel of monoclonal antibodies to estradiol receptor.’ Steroids, 1993, 58, 4-12
Related websites: https://www.medchemexpress.com/antibodies.html
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