Product Name :
Mouse anti Rat Adrenergic Receptor
Description :
| Clone 5G10 | Isotype IgG1 | Product Type Monoclonal Antibody | Units 100 µg | Host Mouse | Species Reactivity Mouse Rat | Application Immunocytochemistry Western Blotting
Background :
Mouse monoclonal antibody raised to a synthetic peptide corresponding to the third intracellular loop of the rat alpha 2b adrenergic receptor.
Source :
The rat alpha 2b adrenergic receptor is a G protein coupled receptor which acts through Gi2 and is responsible for Cellular communication and signal transduction. These receptors are linked to pertussis toxin-sensitive G proteins. Agonist binding leads to inhibition of adenylyl cyclase, inhibition of Ca(2+) channels and activation of K(+) channels. Recently, three distinct subtypes of alpha(2)-AR were described, alpha(2A)-AR, alpha(2B)-AR and alpha(2C)-AR. At present, it is unknown which of these alpha(2)-AR subtype(s) may regulate insulin secretion. <
Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Protein A/G Chromatography Concentration: See vial for concentration
Specificity :
Applications :
Antibody detects alpha2B Adrenergic Receptors in Western blots (1-5 µg/ml) and immunocytochemistry. Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting Positive Control: MDCK cells transfected with rat alpha2B adrenergic receptors.
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Ozawa, Y, et al. ‘Alpha2-adrenergic receptor subtype alterations in the brainstem in the sudden infant death syndrome.’ Early Hum Dev 2003, 75 Suppl, S129-38 2. Yun, J., et al. ‘Gene expression profile of neurodegeneration induced by alpha1B-adrenergic receptor overactivity: NMDA/GABAA dysregulation and apoptosis.’ Brain 2003, Pt 12, 2667-81 3. Peterhoff, M., et al. ‘Inhibition of insulin secretion via distinct signaling pathways in alpha2-adrenoceptor knockout mice.’ Eur J Endocrinol 2003, 4, 343-50 4. Paris, A., et al. ‘Activation of alpha 2B-adrenoceptors mediates the cardiovascular effects of etomidate.’ Anesthesiology 2003, 99, 4, 889-95 5. Brady, AE, et al. ‘Spinophilin stabilizes cell surface expression of alpha 2B-adrenergic receptors.’ J Biol Chem 2003, 278, 34, 32405-12 6. Savola, JM, et al. ‘Fipamezole (JP-1730) is a potent alpha2 adrenergic receptor antagonist that reduces levodopa-induced dyskinesia in the MPTP-lesioned primate model of Parkinson’s disease.’ Mov Disord 2003, 8, 872-83 7. Sivenius, K, et al. ‘A deletion in the alpha2B-adrenergic receptor gene and autonomic nervous function in central obesity.’ Obes Res 2003, 8, 962-70 8. Ge, H, et al. ‘Constitutive precoupling to G(i) and increased agonist potency in the alpha(2B)-adrenoceptor.’ Biochem Biophys Res Commun 2003, 306, 4, 959-65 9. Sykiotis GP, et al. ‘The alpha2B adrenergic receptor deletion/insertion polymorphism in morbid obesity.’ Clin Auton Res 2003, 3, 203-7 10. Feldman J, Bousquet P. ‘Role of cental alpha2B-adrenergic receptors in blood pressure control and hypertension.’ J Hypertens 2003, 5, 871-2 11. Willems EW, et al. ‘Possible role of alpha-adrenoceptor subtypes in acute migraine therapy.’ Cephalalgia 2003, 4, 245-57
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