Product Name :
Mouse anti Rat SNAP25
Description :
| Clone S-7B8 | Isotype IgG1 | Product Type Monoclonal Antibody | Units 100 µg | Host Mouse | Species Reactivity Chicken Cow Human Monkey Mouse Rat | Application Immunocytochemistry Immunohistochemistry (paraffin) Western Blotting
Background :
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with rat brain synaptosomal membranes and mouse NS1 cells.
Source :
SNAP25 (Synaptosome-Associated Protein of 25 kDa) is a membrane bound, pre-synaptic nerve terminal protein that plays an essential role in synaptic vesicle fusion and exocytosis. It is involved in the molecular regulation of neurotransmitter release. It is thought to play an important role in the synaptic function of specific neuronal systems. SNAP25 associates with proteins involved in vesicle docking and membrane fusion. SNAP25 exists as two alternatively spliced isoforms, SNAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells. These two isoforms differ by 9 amino acids in the central portion of the protein. Synonyms: Synaptosome-Associated Protein of 25 kDa <
Product :
Provided as solution in phosphate buffered saline with 0.08% sodium azide Product Form: Unconjugated Purification Method: Protein A/G Chromatography Concentration: See vial for concentration
Specificity :
Applications :
Antibody can be used for Western blotting (1-5 µg/ml), immunocytochemistry and immunohistochemistry on paraformaldehyde fixed CNS tissues (1-5 µg/ml). Does not stain reduced proteins. Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting Positive Control: Cerebellum
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Ritchie, T.C., et al. ‘A nerve terminal protein with a selective distribution in spinal cord and brain.’ J. Neurosci. 1989: 9, 2697-2709 2. Zhao, N., et al. ‘Cloning and sequence analysis of the human SNAP25 cDNA.’ Gene 1994: 145, 313-314 3. McMahon , H.T., et al. ‘Synaptic core complex of synaptobrevin, syntaxin and SNAP25 forms high affinity alpha-SNAP binding site.’ J. Biol. Chem. 1995: 270, 2213-2217 4. Gerona, R.R., et al. ‘The C terminus of SNAP25 is essential for Ca(2+)-dependent binding of synaptotagmin to SNARE complexes.’ J. Biol. Chem. 2000: 275, 6328-6336
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
TRAF2 Antibody
LIAS Antibody
Shh Antibody: Shh Antibody is an unconjugated, approximately 19/47 kDa, rabbit-derived, anti-Shh polyclonal antibody. Shh Antibody can be used for: WB, ELISA, IHC-P, IHC-F, IF expriments in human, mouse, rat, and predicted: chicken, cow, horse, rabbit background without labeling.