Product Name :
Mouse anti Yeast GAL-4 DNA Binding Domain
Description :
| Clone 8C-1 | Isotype IgG1 | Product Type Monoclonal Antibody | Units 100 µg | Host Mouse | Species Reactivity Yeast | Application Immunoprecipitation Western Blotting
Background :
Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells.
Source :
Binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription. Synonyms: Mouse anti GAL-4 DNA Binding region, gal4, gal 4 yeast <
Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Protein A/G Chromatography Concentration: See vial for concentration
Specificity :
Applications :
Recognizes a 106 kDa protein by Western blot in yeast cell extracts corresponding to amino acids 1-147 of the GAL4 DNA binding region. Western blot at 1-10 µg/ml. Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. GAL4 is regulated by a glucose-responsive functional domain. Stone, G. & Sadowski, I. EMBO J. 1993, 12, 1375-1385. 2. An amino-terminal fragment of GAL4 binds DNA as a dimer. Carey, M., et al. J. Mol. Biol. 1989, 209, 423-432. 3. The DNA binding domains of the yeast Gal4 and human c-Jun transcription factors interact through the zinc-finger and bZIP motifs. Sollerbrant, K., et al. Nucleic Acids Res. 1995, 23, 588-594.
Related websites: https://www.medchemexpress.com/antibodies.html
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