Product Name :
Rabbit anti Bovine albumin, conjugated with FITC
Description :
| Clone Polyclonal | Units 1 ml | Host Rabbit
Background :
Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from bovine serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Source :
The defined antibody specificity is directed to albumin as tested against bovine sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against bovine serum and the purified albumin. As reagent for the direct detection of albumin in bovine cells, tissues and body fluids in immunofluorescence techniques. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. <
Product :
9P Product Form: FITC Formulation: Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Specificity :
Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Bovine albumin. Species Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of crossreactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum has been tested in double radial immunodiffusion with the following results: Cat – Chicken – Dog + Duck – Goat ++ Guinea Pig ++ Hamster + Horse + Human + Monkey + Mouse ++ pigeon – Rabbit – Rat ± Sheep – Swine + A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.
Applications :
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
Storage :
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
Related websites: https://www.medchemexpress.com/antibodies.html
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