Product Name :
Rabbit anti Human SMO (Smoothened Homolog)
Description :
| Isotype IgG | Product Type Polyclonal Antibody | Units 100 µg | Host Rabbit | Species Reactivity Human | Application Western Blotting
Background :
Immunogen: Synthetic peptide derived from the human SMO protein
Source :
SMO is a G protein-coupled receptor (GPCR) that putatively associates with the patched protein (PTCH) to transduce the hedgehog’s proteins signal. The binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). In basal cell carcinoma there are two somatic mutations SMO, D473-> H473 and R562 ->Q562. Synonyms: Smoothened homolog [Precursor], SMO, Gx protein, SMO, SMOH <
Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Ammonium Sulfate Precipitation Concentration: See vial for concentration
Specificity :
Applications :
Antibody can be used for Western blotting (1-5µg/ml). Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting Positive Control: Brain
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Stone, D.M., et al. The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog.; Nature 384:129-134(1996). 2. Xie, J., et al. Activating Smoothened mutations in sporadic basal-cell carcinoma.; Nature 391:90-92(1998). 3. Liu, Y., et al. ‘Fused-Costal2 protein complex regulates Hedgehog-induced Smo phosphorylation and cell-surface accumulation.’ Genes Dev. 2007 Aug 1;21(15):1949-63. 4. Moraea, R.C., et al. ‘Constitutive activation of smoothened (SMO) in mammary glands of transgenic mice leads to increased proliferation, altered differentiation and ductal dysplasia.’ Development. 2007 Mar;134(6):1231-42. 5. Ruiz-Gomez, A., et al. ‘The cell biology of Smo signalling and its relationships with GPCRs.’ Biochim Biophys Acta. 2007 Apr;1768(4):901-12.
Related websites: https://www.medchemexpress.com/antibodies.html
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