Product Name :
Rabbit anti Rat G Protein b5-D1
Description :
| Isotype IgG | Product Type Polyclonal Antibody | Units 100 µg | Host Rabbit | Species Reactivity Human Murine Rat | Application Western Blotting
Background :
Rabbits were immunized with a unique peptide sequence derived from the D1 blade of the G 5 protein
Source :
The G beta5 protein is a G protein subunit present predominantly in the central nervous system. This protein appears to be expressed in two forms, one found in the brain (39 kDa) and one found in the retina (44 kDa). It has also been found that Gb5 associates tightly with RGS7, a member of the superfamily of negative regulators of G protein signaling. These complexes have been localized to most regions of the brain, whereas G 1-4 exhibit no observable colocalization with RGS7, despite their widespread codistribution throughout similar neuronal networks. <
Product :
This antibody is specific for a peptide sequence within the D1 blade which is not found in the G betas 1-4. Provided as solution in phosphate buffered saline with 0.08% sodium azide. Product Form: Unconjugated Purification Method: Ammonium Sulfate Precipitation Concentration: See vial for concentration
Specificity :
Applications :
Antibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting Positive Control: Plasma membranes from rat cerebrum
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Liang, J.J., et al. ‘RGS7 complex formation and colocalization with the Gbeta5 subunit in the adult rat and influence on Gbeta5gamma2-mediated PLCbeta signaling.’ J. Neurosci. Res. 2000: 60, 58-64 2. Cabrera, J.L., et al. ‘Identification of the Gbeta5-RGS7 protein complex in the retina.’ Biochem Biophys Res Commun. 1998: 249, 898-902 3. Watson, A.J., et al. ‘A novel form of the G protein beta subunit Gbeta5 is specifically expressed in the vertebrate retina.’ J. Biol. Chem. 1996: 271, 28154-28160
Related websites: https://www.medchemexpress.com/antibodies.html
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