Product Name :
Sheep anti Mouse LFC
Description :
| Isotype IgG | Product Type Polyclonal Antibody | Units 250 µg | Host Sheep | Species Reactivity Mouse | Application Immunofluorescence Immunoprecipitation Western Blotting
Background :
Immunogen: Antibody developed using protein corresponding to amino acids 2 to 447 of the Lfc protein fused to GST.
Source :
Lfc is a 118 kDa protein discovered in a screen for oncogenes in NIH 3T3 cells. Lfc is a signaling protein related to Lbc and is part of the Dbl family of proteins. Lfc is a guanine-nucleotide exchange factor (GEF) for the Rho family of small GTPases. Lfc has also been shown to be upstream of the serine-threonine kinase JNK. Lfc has also been shown to mediate the activation of the Rac signaling pathway. Synonyms: Rho guanine nucleotide exchange factor 2, ARHGEF2 <
Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Ammonium Sulfate Precipitation Concentration: See vial for concentration
Specificity :
Applications :
Detects Lfc in mouse tissues by immunoprecipitation-Western blot at 4 µg/ml. Detects a 118 kDa band in mouse tissue extracts corresponding to wild-type Lfc. Can also be used in immunofluorescence assays. Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Glaven, J.A., et al. The Dbl-related protein, Lfc, localizes to microtubules and mediates the activation of Rac signaling pathways in cells. J. Biol. Chem. 1999, 274, 2279-2285 2. Glaven, J.A., et al. Lfc and Lsc oncoproteins represent two new guanine nucleotide exchange factors for the Rho GTP-binding protein. J. Biol. Chem. 1996, 271, 27374-27381 3. Whitehead, I., et al. Expression cloning of lfc, a novel oncogene with structural similarities to guanine nucleotide exchange factors and to the regulatory region of protein kinase C. J. Biol. Chem. 1995, 270, 18388-18395
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