Product Name :
Sheep anti Rat SSeCKS – myristylated (PKC) substrate
Description :
| Isotype IgG | Product Type Polyclonal Antibody | Units 250 µg | Host Sheep | Species Reactivity Rat | Application Immunohistochemistry Immunoprecipitation Western Blotting
Background :
Sheep were immunized with a recombinant protein corresponding to amino acids 620-1596 of the SSeCKS protein.
Source :
Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation and proliferation, yet little is known about downstream substrates or their roles in normal or diseased states. SSeCKS, a novel protein kinase C substrate originally isolated as a src-suppressed negative mitogenic regulator in fibroblasts, controls actinbased cytoskeletal architecture and scaffolds key signaling kinases such as protein kinase C and protein kinase A. Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation. A role for SSeCKS, a PKA/PKC scaffolding protein, has been implicated during the process of spermiogenesis and in the actin-based stellate morphology of mesangial cells Synonyms: SSeCKS; A-kinase anchor protein 12; AKAP12; src-suppressed C kinase substrate <
Product :
Provided as solution in phosphate buffered saline with 0.08% sodium azide Product Form: Unconjugated Purification Method: Ammonium Sulfate Precipitation Concentration: See vial for concentration
Specificity :
Applications :
Antibody can be used for Western blot (2-5 µg/ml), immunohistochemistry and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Western Blotting Positive Control: Rat aortic smooth muscle
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Lin, X., et al. ‘A novel src- and ras-suppressed protein kinase C substrate with cytoskeletal architecture.’ J. Biol. Chem. 1996, 271, 28430-28438 2. Siegel, S.M., et al. ‘SSeCKS immunolabeling in rat primary sensory neurons.’ Brain Res. 2002, 926, 126-136 3. Nelson, P.J., et al. ‘Involvement of the protein kinase C substrate, SSeCKS, in the actin-based stellate morphology of mesangial cells.’ J. Cell. Sci. 1999, 112, 361-370
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
PKC beta 1 Rabbit mAb MedChemExpress
NFAT1 Rabbit mAb Autophagy
RSK1 p90 Antibody (YA088): RSK1 p90 Antibody (YA088) is a non-conjugated and Rabbit origined monoclonal antibody about 83 kDa, targeting to RSK1 p90. It can be used for WB,ICC,IHC-P,FC assays with tag free, in the background of Human, Mouse, Rat.