Product Name :
Goat anti Human IgM (Fc specific), conjugated with FITC
Description :
| Clone Polyclonal | Product Type Polyclonal Antibody | Units 1 ml | Host Goat | Species Reactivity Human | Application ELISA Immunocytochemistry Immunofluorescence Immunohistochemistry
Background :
Purified IgM prepared from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Source :
The antiserum is reacting with to the Fc subunit of IgM. It does not react with other immunoglobulins or any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgM. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected..This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. <
Product :
FITC-coupled goat F(ab’)2 antibodies lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Product Form: FITC Formulation: FITC-coupled goat F(abÕ)2 antibodies lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
Specificity :
Fluorescein isothiocyanate-conjugated F(ab’)2 fragments of polyclonal Goat IgG antibodies to Human IgM, Fc specific Species Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this conjugate has not been tested in detail.
Applications :
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
Storage :
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
Cdk6 Rabbit mAb Epigenetics
Lonigutamab Protein Tyrosine Kinase/RTK
Calpastatin Antibody: Calpastatin Antibody is a non-conjugated and Mouse origined monoclonal antibody about 120 kDa, targeting to Calpastatin. It can be used for WB,ICC,IHC-P assays with tag free, in the background of Human.