Product Name :
Mouse anti Human CD11b
Description :
| Clone F6.2 | Isotype IgG2a | Product Type Single-Color Reagent | Units 100 µg | Host Mouse | Species Reactivity Human | Application Flow Cytometry
Background :
Source :
(Mac-1), CD11b/CD18 complex is a leukocyte adhesion receptor to intercellular adhesion molecules (ICAM’s). CR3 alpha chain (M.W. 165,000) is on virtually all peripheral blood neutrophils, eosinophils, monocytes and approximately 30% of peripheral blood lymphocytes. Synonyms: CD11b <
Product :
Product Form: Unconjugated Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Protein A/G Chromatography Concentration: See vial for concentration
Specificity :
Applications :
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8° C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. Functional Analysis: Flow Cytometry Staining
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Room Temperature
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1.Structure and function of the leukocyte adhesion molecules CD11/CD18. Arnaout, M.A., Blood . 1990 Mar; 75(5):1037-50. 2. Leukocyte adhesion to endothelial cells. Wautier, ,J.L., Sediadi, H., Vilette, d., Weill, D., Wautier, M.P.. Biorheology . 1990:27(34): 425-32. 3. Flow cytometric analysis of neutrophil subsets in thermally injured patients developing infection. Babcock, G.F., Alexander, J.W., Warden, G.d.. Clin. Immunol. Immunopathol. 1990 Ja;54(1):117-25. 4. Leukocyte Adhesion Deficiency: an inherited defect in Mac-1,LFA-1, and p150,95 glycoproteins. Anderson, D.C. and Springer, T.A.. Ann. Rev. Med. 1987;38:175. 5. Two pathways of CD11b/CD18-mediated neutrophil aggregation with different involvedment of protein kinase C-dependent phosphorylation. Merrill, J.T., Slade, S.G., Weissman, G., Winchester, R., Buyon, J.P.. J. Immunology. 1990 Oct.1;145(8):2608-15. 6. Tumor necrosis factor-induce degranulation in adherent human neutrophils is dependent on CD11b/CD18-integrin-triggered oscillations of cytosolic free Ca2+. Richter, J., Ng-Sikorski, J., Olsson, I., Andersson, T.. Proc. Natl. Acad. Sci. USA 1990 De;87(23):9472-6.
Related websites: https://www.medchemexpress.com/antibodies.html
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