Product Name :
Rabbit anti Human 5T4 Oncofetal Trophoblast glycoprotein
Description :
| Isotype Ig | Product Type Antigen Immunoaffinity Purified Polyclonal | Units 100 µg | Host Rabbit | Species Reactivity Human | Application ELISA Immunohistochemistry Western Blotting
Background :
Immunogen: Synthetic peptide derived from human 5T4 oncofetal antigen protein
Source :
5T4 is a N-glycosylated transmembrane 72 kDa (420 AA) glycoprotein containing seven leucine-rich repeat regions. 5T4 is often referred to as an oncofetal antigen due to its expression in fetal trophoblast where it was first discovered or trophoblast glycoprotein (TPBG). 5T4 oncofetal antigen is defined by a monoclonal antibody originally raised against human placental trophoblast and which recognizes a 72 kD glycoprotein expressed in a variety of different carcinomas but detected only at very low levels in normal epithelia. Patterns of expression of 5T4 oncofetal antigen in colorectal carcinomas indicate a significant association with tumor metastasis. By IHC, 71% of the carcinomas demonstrate positive 5T4 immunoreactivity in adenocarcinoma cells and/or associated stromal tissue.5T4 is found in tumors including the colorectal, ovarian, and gastric. Its expression is used as a prognostic aid in these cases. 5T4 is widespread in malignant tumors throughout the various stages of their development. In assessing the 5T4 phenotype it has been determined that there is a significant correlation between 5T4 expression and advanced stage of disease (FIGO stages III and IV) (P Synonyms: Trophoblast glycoprotein, TPBG, 5T4 oncofetal antigen, 5T4 oncotrophoblast glycoprotein, 5T4 oncofetal trophoblast glycoprotein, M6P1 <
Product :
Product Form: Affinity Purified Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Antigen Immunoaffinity Purification Concentration: See vial for concentration
Specificity :
Applications :
This antibody can be used for immunohistochemistry on formalin/paraffin sections (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Immunohistochemistry Positive Control: Ovarian Cancer, gastric cancer, colorectal cancer.
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Yoshihara, S., et al. ‘5T4 glycoprotein regulates the sensory input-dependent development of a specific subtype of newborn interneurons in the mouse olfactory bulb.’ J. Neurosci. 2012, 32, 2217-2226. 2. Southgate, T.D., et al. ‘CXCR4 mediated chemotaxis is regulated by 5T4 oncofetal glycoprotein in mouse embryonic cells.’ PLoS One 2010, 5, e9982. 3. Shingler, W.H., et al. ‘Identification and functional validation of MHC class I epitopes in the tumor-associated antigen 5T4.’ Int. Immunol. 2008, 20, 1057-1066.
Related websites: https://www.medchemexpress.com/antibodies.html
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