Product Name :
Rabbit anti Human DNA Topoisomerase 1
Description :
| Isotype Ig | Product Type Antigen Immunoaffinity Purified Polyclonal | Units 100 µg | Host Rabbit | Species Reactivity Human | Application Immunohistochemistry (paraffin)
Background :
Immunogen: Synthetic peptide made to human DNA Topoisomerase 1 [(c)-EVQATDREENKQIALGTSKLN] recognizing a specific sequence within amino acids 692-766.
Source :
DNA Topoisomerase 1 releases the supercoiling and torsional tension of DNA introduced during DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. It introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3′-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5′-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5′-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. <
Product :
Product Form: Affinity Purified Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Antigen Immunoaffinity Purification Concentration: See vial for concentration
Specificity :
Applications :
Antibody can be used for immunohistochemistry at 5-10 µg/ml on paraffin-embedded tissues. Optimal concentration should be evaluated by serial dilutions. Positive Control: Found in Endothelial cells.
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Oddou, P., et al. ‘Monoclonal antibodies neutralizing mammalian DNA topoisomerase I activity.’ Eur. J. Biochem. 1988, 177, 523-529 2. Redinbo, M.R., et al. ‘Novel insights into catalytic mechanism from a crystal structure of human topoisomerase I in complex with DNA.’ Biochemistry 2000, 39, 6832-6840 3. Eisenreich, A., et al. ‘Cdc2-like kinases and DNA topoisomerase I regulate alternative splicing of tissue factor in human endothelial cells.’ Circ. Res. 2009, 104, 589-599
Related websites: https://www.medchemexpress.com/antibodies.html
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