Product Name :
Rabbit anti Human lysozyme
Description :
| Clone Polyclonal | Product Type Polyclonal Antibody | Units 10 mg | Host Rabbit | Species Reactivity Human
Background :
Lysozyme, a bacteriolytic enzyme, has a molecular weight of about 15,000. Its concentration in plasma is low; in milk the concentration is reported to be between 5 and 220 mg/ml. Also tears contain a considerable amount of lysozyme.Highly purified recombinant human lysozyme was used as immunogen. Freund’s complete adjuvant is used in the first step of the immunization procedure. Immunogen: Highly purified recombinant human lysozyme was used as immunogen.
Source :
The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum. The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. <
Product :
Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Specificity :
IgG fraction of polyclonal Rabbit antiSerum to Human lysozyme. Species Reactivity: The antiSerum does not cross-react with any other component of Human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
Applications :
Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided.
Storage :
The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Shipping Conditions: Ship at ambient temperature.
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
Histone H3 Antibody
Cy7-conjugated AffiniPure Goat Anti-Mouse IgG H&L
Glutathione Synthetase Antibody: Glutathione Synthetase Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 52 kDa, targeting to Glutathione Synthetase. It can be used for WB,ICC,IHC-P,FC assays with tag free, in the background of Human, Mouse, Rat.