Product Name :
CD68 (KP1) Macrophages, human
Description :
| Clone KP1 | Isotype IgG1, k | Units 0.5 ml | Host Mouse | Application Immunohistochemistry (frozen) Immunohistochemistry (paraffin)
Background :
Immunogen: Subcellular fraction of human alveolar macrophages
Source :
The antibody KP1 is well suited for the detection of tissue macrophages including Kupffer cells and spleen macrophages. CD68 is expressed on macrophages and monocytes. KP-1 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. KP-1 reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Tumors of lymphoid origin are usually not stained. CD68 transmembran glycoprotein MW 110 kDa, localised in macrophages. <
Product :
Purified antibody with 0.2% BSA and 15 mM sodium azide as preservative Purification Method: Purified antibody with 0.2% BSA and 15 mM sodium azide as preservative Secondary Reagents: We recommend the use of BIOLOGO’s Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Specificity :
Species Reactivity: Human, monkey, cat and rat. Does not react with pig, dog and chicken
Applications :
IHC(C, P) Incubation Time: 60 min at RT Working Concentration: (liquid conc.) 1:100-1:200 Pre-Treatment: Unmasking with Pronase (Art. No. DE110) Positive Control: Tonsil, spleen, lymph node
Storage :
2-8°C
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Fukuda M. (1991) Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficing. J. Biol. Chem. 266; 21327-21330. 2. Holness C.I. and Simmons D.L. (1993) Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood 81; 1607-1613. 3. Pulford KAF, Rigney EM, Micklem KJ, Jones M, Stross WP, Gatter KC, et al. (1989) KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. J Clin Pathol;42:414-21. 4. Warnke RA, Pulford KAF, Pallesen G, Ralfkiaer E, Brown DC, Gatter KC, et al. (1989) Diagnosis of myelomonocytic and macrophage neoplasms in routinely processed tissue biopsies with monoclonal antibody KP1. Am J Pathol;135:1089-95.
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
Disitamab Antibody-drug Conjugate/ADC Related
Olokizumab Autophagy
Adipose Triglyceride Lipase Antibody: Adipose Triglyceride Lipase Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 55 kDa, targeting to Adipose Triglyceride Lipase. It can be used for WB assays with tag free, in the background of Human, Mouse, Rat.