Product Name :
Goat anti Rat IgG1 IgG2a IgG2b IgG2c (Fc specific), conjugated with TRITC
Description :
| Clone Polyclonal | Units 1 ml | Host Goat
Background :
Purified normal IgG, including all subclasses, isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Source :
The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum containsantibodies to subclass-specific determinants as well as to determinants shared by two or more subclassesof IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserumconcentrations against normal rat plasma, serum, and immunoglobulin fractions, no reaction is obtained withIgA, IgM and IgG/Fab fragments or any other serum protein. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. <
Product :
TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Product Form: TRITC Formulation: TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Specificity :
Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Rat IgG, Fc specific. Species Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this product has not been tested in detail.
Applications :
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
Storage :
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
Cetrelimab manufacturer
USP7 (Y20P42) Mouse mAb custom synthesis
STAT3 Antibody (YA056): STAT3 Antibody (YA056) is a non-conjugated and Rabbit origined monoclonal antibody about 88 kDa, targeting to STAT3. It can be used for WB,ICC/IF,IHC-P,IP assays with tag free, in the background of Human, Mouse, Rat.