Product Name :
Rabbit anti-WT1 Polyclonal Antibody
Synonym :
Wilms tumor protein; WT33
Host :
Rabbit
Species Reactivity:
Human, Mouse, Rat
Specificity :
WT1 Antibody detects endogenous levels of total WT1.
Predicted Reactivity:
Applications :
WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
Immunogen:
A synthesized peptide derived from human WT1, corresponding to a region within N-terminal amino acids.
Concentration :
1mg/ml
Purification :
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality:
Monoclonal antibody
Storage Temp.:
Store at -20 ° C for one yearAvoid repeated freeze/that cycles
Research areas :
Background :
Wilms tumor 1 (WT1) gene encodes a zinc finger transcription factor (WT1) that regulates genes involved in proliferation, differentiation and apoptosis, while it plays a central role in the embryogenesis of many tissues including the urogenital system, heart, and central nervous system.Although predominantly expressed in nucleus in normal cells, WT1 shuttles between the nuclear and cytoplasmic compartments. WT1 is detected in functional polysomes; interacts with the splice factor U2AF65; and is associated with nuclear mRNP particles in fetal kidney cells. In WT1‐expressing tumors, WT1 is mostly expressed in the cytoplasm and interacts with the actin cytoskeleton.
UniProt :
P19544
Additional information:
Product Details FAQ Citations(3) Video Pictures Documents |Overview |Description This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm’s tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Oct 2010] |Synonym Wilms tumor protein; WT33 |Host Rabbit |Specificity WT1 Antibody detects endogenous levels of total WT1. |Species Reactivity Human, Mouse, Rat |Applications WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000 |Immunogen A synthesized peptide derived from human WT1, corresponding to a region within N-terminal amino acids. |Properties |Form liquid |Concentration 1mg/ml |Purification The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin . |Clonality Monoclonal antibody |Clone R076 |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |Target |Background Wilms tumor 1 (WT1) gene encodes a zinc finger transcription factor (WT1) that regulates genes involved in proliferation, differentiation and apoptosis, while it plays a central role in the embryogenesis of many tissues including the urogenital system, heart, and central nervous system.Although predominantly expressed in nucleus in normal cells, WT1 shuttles between the nuclear and cytoplasmic compartments. WT1 is detected in functional polysomes; interacts with the splice factor U2AF65; and is associated with nuclear mRNP particles in fetal kidney cells. In WT1‐expressing tumors, WT1 is mostly expressed in the cytoplasm and interacts with the actin cytoskeleton. |tissuespecificity Expressed in the kidney and a subset of hematopoietic cells. |Cellular localization Cytosol;Nucleus; |UniProt P19544 | Western blot analysis of kidney cell lysateusing WT1 antibody. |Tips:This product is for research use only. Not for use in diagnostic prodcedures.
Rabbit anti-WT1 Recombinant Monoclonal Antibody(R076)
Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.Related websites: https://www.medchemexpress.com/antibodies.html
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