Tors SOST and DKK1. Biochem Biophys Res Commun 414:32630. sixteen. Wingerter S, Tucci M, Bumgardner J, Benghuzzi H (2007) Evaluation of short-term healing following sustained delivery of osteoinductive agents in a rat femur drill defect model. Biomed Sci Instrum 43:18893. 17. Winnier G, Blessing M, Labosky PA, Hogan BL (1995) Bone morphogenetic protein-4 is required for mesoderm formation and patterning in the mouse. Genes Dev 9:2105116. 18. Zhang H, Bradley A (1996) Mice deficient for BMP2 are nonviable and have defects in amnion/chorion and cardiac growth. Improvement 122:2977986.19. Seeherman HJ, Li XJ, Bouxsein ML, Wozney JM (2010) rhBMP-2 induces transient bone resorption followed by bone formation in a nonhuman primate core-defect model. J Bone Joint Surg Am 92:41126. 20. Dewulf N, et al. (1995) Distinct spatial and temporal DP Inhibitor Source expression patterns of two form I receptors for bone morphogenetic proteins all through mouse embryogenesis. Endocrinology 136:2652663. 21. Mishina Y, Suzuki A, Ueno N, Behringer RR (1995) Bmpr encodes a variety I bone morphogenetic protein receptor that may be necessary for gastrulation through mouse embryogenesis. Genes Dev 9:3027037. 22. Mao B, et al. (2001) LDL-receptor-related protein 6 is a receptor for Dickkopf proteins. Nature 411:32125. 23. Brott BK, Sokol SY (2002) Regulation of Wnt/LRP signaling by distinct domains of Dickkopf proteins. Mol Cell Biol 22:6100110. 24. Tian E, et al. (2003) The purpose of your Wnt-signaling antagonist DKK1 within the development of osteolytic lesions in a number of myeloma. N Engl J Med 349:2483494. 25. Fujita K, Janz S (2007) Attenuation of WNT signaling by DKK-1 and -2 regulates BMP2induced osteoblast differentiation and expression of OPG, RANKL and M-CSF. Mol Cancer 6:71. 26. Mukhopadhyay M, et al. (2001) Caspase 7 Inhibitor Formulation Dickkopf1 is required for embryonic head induction and limb morphogenesis while in the mouse. Dev Cell one:42334. 27. Grotewold L, R her U (2002) The Wnt antagonist Dickkopf-1 is regulated by Bmp signaling and c-Jun and modulates programmed cell death. EMBO J 21:96675. 28. Okamoto M, Murai J, Yoshikawa H, Tsumaki N (2006) Bone morphogenetic proteins in bone stimulate osteoclasts and osteoblasts through bone growth. J Bone Miner Res 21:1022033. 29. Canalis E, Parker K, Zanotti S (2012) Gremlin1 is required for skeletal advancement and postnatal skeletal homeostasis. J Cell Physiol 227:26977. thirty. Canalis E, Brunet LJ, Parker K, Zanotti S (2012) Conditional inactivation of noggin from the postnatal skeleton brings about osteopenia. Endocrinology 153:1616626. 31. Bouxsein ML, et al. (2010) Tips for assessment of bone microstructure in rodents working with micro-computed tomography. J Bone Miner Res 25:1468486. 32. Glatt V, Canalis E, Stadmeyer L, Bouxsein ML (2007) Age-related changes in trabecular architecture differ in female and male C57BL/6J mice. J Bone Miner Res 22:1197207. 33. Leung CK, Lam FK (1996) Functionality examination to get a class of iterative image thresholding algorithms. Pattern Recognit 29:1523530. 34. Ridler TW, Calvard S (1978) Picture thresholding utilizing an iterative selection technique. IEEE Trans Syst Man Cybern eight:63032. 35. Heath DJ, et al. (2009) Inhibiting Dickkopf-1 (Dkk1) removes suppression of bone formation and prevents the development of osteolytic bone ailment in various myeloma. J Bone Miner Res 24:42536. 36. Pearsall RS, et al. (2008) A soluble activin style IIA receptor induces bone formation and improves skeletal integrity. Proc Natl Acad Sci USA 105:7082087.12212 www.pnas.org/.