The bars symbolize the normalized luciferase activity (mean6s.d., n = 5). (F) miRNA qRT-PCR analysis of miRNAs pertaining to the miRNA 17-5p-92 cluster in Tet-21/N untreated (two Dox) or taken care of with doxycycline for ninety six h (+ Dox)

To display that miR-seventeen-5p directly regulates p21 expression by binding to its 39 UTR, we cloned the p21 39 UTR into the pGL3-Promoter vector, downstream the luciferase gene (pGL3Prom-p21UTR-wt). As…