The animals have been then injected with GBR12909 (5 mg/kg) and samples were gathered for 20 min throughout a pretreatment period of time of eighty min
DAT phosphorylation in D5R-KO mice. DAT proteins had been immunoprecipitated from entire brain lysates and ended up then immunoblotted with anti-phosphothreonine and anti-DAT antibodies. (a) Upper: Agent Western blot of…