The structure of Cy3-labeled DNA fragments. B: The localization of Cy3-DNA fragments co-injected with NLS-I-SceI mRNA. C: The localization of Cy3-DNA fragments co-injected with the indigenous I-SceI nuclease
The dynamics of transgene expression in the embryos cytoplasmically co-injected with NLS-I-SceI mRNA and circular transgene plasmid was very similar to that in embryos subjected to pronuclear microinjection only with…